hLDLR-TEV-TwinStrep and 438-A-hLDLRecto-TEV-TwinStrep plasmids for expression of human insulin receptor and LDL binding ectodomain in insect cells
| Název česky | Plazmidy hLDLR-TEV-TwinStrep a 438-A-hLDLRecto-TEV-TwinStrep pro expresi lidského LDL receptoru a jeho LDL-vazebné extracelulární domény v hmyzích buňkách |
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| Autoři | |
| Rok publikování | 2025 |
| Druh | Technicky realizované výsledky (prototyp, funkční vzorek) |
| Fakulta / Pracoviště MU | |
| Přiložené soubory | |
| Popis | The functional sample consists of donor plasmids enabling expression of full-length human low-density lipoprotein receptor or its LDL-binding extracellular domain in insect cells using the Bac-to-Bac baculovirus system. The constructs include a C-terminal TEV-cleavable Twin-Strep tag and are optimized for preparative purification and downstream structural studies. This work was supported by the project National Institute for Research of Metabolic and Cardiovascular Diseases (Program EXCELES, ID Project No. LX22NPO5104), funded by the European Union-Next Generation EU. |
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