Deciphering the Tau/14-3-3 Interaction via Artificial Intelligence and Chemical Cross-Linking

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Authors

IĽKOVIČOVÁ Lucia MILOTOVÁ Vendula HRITZ Jozef

Year of publication 2026
Type Appeared in Conference without Proceedings
MU Faculty or unit

Central European Institute of Technology

Citation
Description The aggregation of tau protein plays a crucial role in several neurodegenerative diseases, including Alzheimer’s disease, Chronic traumatic encephalopathy and others. The diseases are characterized by the presence of tau fibrils with specific morphologies. Different phosphorylation patterns have been observed in diseases, indicating that morphology is dependent on the posttranslational modifications. With the release of AlphaFold3 allowing the structure prediction of posttranslationally modified proteins, we examined the differences in the predicted structures of several tau constructs with different phosphorylations. Still, the exact cause and mechanism of tau aggregation remain unknown. However, the interaction with 14-3-3 proteins modulates the aggregation properties of tau protein. On this poster, we also evaluate models of tau/14-3-3 interactions validated by chemical cross-linking.
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